Rapid analytical approach for bioprofiling compounds with radical scavenging and antimicrobial activities from seaweeds

Food Chem. 2021 Jan 1:334:127562. doi: 10.1016/j.foodchem.2020.127562. Epub 2020 Jul 17.


Brown seaweeds are traditionally used as food in Asian countries, and they are a valuable source of bioactive compounds. Herein, a novel high-throughput methodological approach was developed for the tracing of compounds with radical scavenging and antimicrobial activities in Saccharina japonica and Undaria pinnatifida methanol extracts. The seaweed metabolites were separated by a novel high-performance thin-layer chromatography method, the bioactive bands were identified by bioautography assays. The bioactive compounds were characterized with ultra-high-performance liquid chromatography coupled with linear trap quadrupole tandem mass spectrometry. Stearidonic, eicosapentaenoic, and arachidonic acids were identified as major components having radical scavenging and antimicrobial activities. The suggested method provides a fast identification and quantification of bioactive compounds in multicomponent biological samples.

Keywords: Bioautography; Gas chromatography-mass spectrometry; Planar chromatography; Seaweeds; Ultra-high performance liquid chromatography-mass spectrometry.

MeSH terms

  • Anti-Infective Agents / analysis*
  • Anti-Infective Agents / chemistry
  • Arachidonic Acids / analysis
  • Arachidonic Acids / pharmacology
  • Chromatography, Thin Layer / methods*
  • Eicosapentaenoic Acid / analysis
  • Eicosapentaenoic Acid / pharmacology
  • Fatty Acids, Omega-3 / analysis
  • Fatty Acids, Omega-3 / pharmacology
  • Free Radical Scavengers / chemistry
  • Free Radical Scavengers / pharmacology
  • Gas Chromatography-Mass Spectrometry
  • High-Throughput Screening Assays / methods
  • Laminaria / chemistry
  • Laminaria / metabolism
  • Seaweed / chemistry*
  • Seaweed / metabolism
  • Tandem Mass Spectrometry / methods*
  • Undaria / chemistry
  • Undaria / metabolism


  • Anti-Infective Agents
  • Arachidonic Acids
  • Fatty Acids, Omega-3
  • Free Radical Scavengers
  • Eicosapentaenoic Acid
  • stearidonic acid