Cell Growth Inhibition of Saponin XII from Dipsacus japonicus Miq. on Acute Myeloid Leukemia Cells

Ba Thi Cham; Nguyen Thi Thuy Linh; Do Thi Thao; Nguyen Thi Hoang Anh; Nguyen Thanh Tam; Bui Kim Anh; Isabella Muscari; Sabrina Adorisio; Tran Van Sung; Trinh Thi Thuy; Domenico V Delfino

doi:10.3390/molecules25153325

Cell Growth Inhibition of Saponin XII from Dipsacus japonicus Miq. on Acute Myeloid Leukemia Cells

Molecules. 2020 Jul 22;25(15):3325. doi: 10.3390/molecules25153325.

Authors

Ba Thi Cham^{1

2}, Nguyen Thi Thuy Linh^{1

2}, Do Thi Thao^{1

3}, Nguyen Thi Hoang Anh^{1

2}, Nguyen Thanh Tam^{1

2}, Bui Kim Anh², Isabella Muscari⁴, Sabrina Adorisio⁵, Tran Van Sung², Trinh Thi Thuy^{1

2}, Domenico V Delfino^{5

6}

Affiliations

¹ Department of Chemistry, Graduate University of Science and Technology, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghia Do, Cau Giay, 100000 Hanoi, Vietnam.
² Department of Natural Products Research, Institute of Chemistry, VAST, 18 Hoang Quoc Viet, Nghia Do, Cau Giay, 100000 Hanoi, Vietnam.
³ Institute of Biotechnology, VAST, 18 Hoang Quoc Viet, Nghia Do, Cau Giay, 100000 Hanoi, Vietnam.
⁴ Section of onco-hematology, Department of Medicine, University of Perugia, 06132 Perugia, Italy.
⁵ Foligno Nursing School, Department of Medicine, University of Perugia, 06132 Perugia, Italy.
⁶ Section of Pharmacology, Department of Medicine, University of Perugia, Piazzale Severi, S. Andrea delle Fratte, 06132 Perugia, Italy.

Abstract

In previous studies, we isolated the known compound saponin XII from the roots of Dipsacus japonicus Miq. Here, we show that this compound reduced the number of acute myeloid leukemia OCI-AML3 cells as evaluated by a hemocytometer. Flow cytometry analyses demonstrated that the reported activity was associated with a significant increase of apoptosis and of cells in the G₀/G₁ phase of the cell cycle, with a decrease of cells in the S and G₂/M phases. Thus, the inhibition of cell growth in OCI-AML3 cells was due to antiproliferative and pro-apoptotic effects. Interestingly, the bioactivity of saponin XII exerted its effect at a concentration as low as 1 µg/mL.

Keywords: Dipsacus japonicus Miq.; OCI-AML cells; antiproliferative activity; cancer; saponin XII.

MeSH terms

Antineoplastic Agents / chemistry*
Antineoplastic Agents / pharmacology
Apoptosis / drug effects
Cell Division / drug effects
Cell Line, Tumor
Cell Proliferation / drug effects
Dipsacaceae / chemistry*
Drug Discovery
Growth Inhibitors / chemistry*
Growth Inhibitors / pharmacology
Humans
Leukemia, Myeloid, Acute / drug therapy*
Plant Extracts / chemistry*
Plant Extracts / pharmacology
Saponins / chemistry*
Saponins / pharmacology

Substances

Antineoplastic Agents
Growth Inhibitors
Plant Extracts
Saponins

Abstract

MeSH terms

Substances

Grants and funding