Abstract
Some long non-coding RNA (lncRNA) genes encode a functional RNA product, whereas others act as DNA elements or via the act of transcription . We describe here a ribozyme-based approach to deplete an endogenous lncRNA in mouse embryonic stem cells, with minimal disruption of its gene. This enables the role of the lncRNA product to be tested.
Keywords:
Genome editing; Long non-coding RNA; RNA; Ribozyme; lincRNA; lncRNA.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
CRISPR-Cas Systems
-
Embryonic Stem Cells / enzymology
-
Embryonic Stem Cells / metabolism*
-
Gene Editing / methods*
-
Mice
-
Nucleic Acid Conformation
-
RNA, Catalytic / genetics*
-
RNA, Catalytic / metabolism
-
RNA, Long Noncoding / genetics*
-
RNA, Long Noncoding / metabolism
-
RNA, Viral / genetics*
-
Recombination, Genetic
Substances
-
RNA, Catalytic
-
RNA, Long Noncoding
-
RNA, Viral