Long Non-coding RNA Depletion Using Self-Cleaving Ribozymes

Methods Mol Biol. 2021:2167:287-301. doi: 10.1007/978-1-0716-0716-9_16.

Abstract

Some long non-coding RNA (lncRNA) genes encode a functional RNA product, whereas others act as DNA elements or via the act of transcription . We describe here a ribozyme-based approach to deplete an endogenous lncRNA in mouse embryonic stem cells, with minimal disruption of its gene. This enables the role of the lncRNA product to be tested.

Keywords: Genome editing; Long non-coding RNA; RNA; Ribozyme; lincRNA; lncRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CRISPR-Cas Systems
  • Embryonic Stem Cells / enzymology
  • Embryonic Stem Cells / metabolism*
  • Gene Editing / methods*
  • Mice
  • Nucleic Acid Conformation
  • RNA, Catalytic / genetics*
  • RNA, Catalytic / metabolism
  • RNA, Long Noncoding / genetics*
  • RNA, Long Noncoding / metabolism
  • RNA, Viral / genetics*
  • Recombination, Genetic

Substances

  • RNA, Catalytic
  • RNA, Long Noncoding
  • RNA, Viral