Low-temperature study of the A-DNA fragment d(GGGCGCCC)

Acta Crystallogr B. 1988 Dec 1:44 ( Pt 6):625-8. doi: 10.1107/s0108768188004732.

Abstract

The structure of the A-type duplex d(GGGCGCCC) was determined from data measured at 115 K to 2.0 A resolution. The space group, P4(3)2(1)2, is the same as for the 293 K structure; cell dimensions are a = 42.74 (4), c = 24.57 (1) A; R = 0.21 for 1694 observed reflections. The conformation and hydration are similar at the two temperatures. The average displacement parameters (B) for bases, sugars and phosphates all decrease by about 9 A2 relative to those found at 293 K. The individual values of B1/2 are linearly related to the distance from the molecular center of mass.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Base Sequence
  • DNA*
  • Freezing
  • Nucleic Acid Conformation*
  • Oligodeoxyribonucleotides*
  • X-Ray Diffraction / methods

Substances

  • Oligodeoxyribonucleotides
  • DNA