Constant-moderate versus high-intensity interval training on heart adiponectin levels in high-fat fed mice: a preventive and treatment approach

Arch Physiol Biochem. 2023 Feb;129(1):41-45. doi: 10.1080/13813455.2020.1797098. Epub 2020 Jul 25.


Context: Research has described that adiponectin plays a key role in cardiomyocytes metabolism, however, the effects of exercise during obesity on cardiac adiponectin levels is unclear.

Objective: To investigate the effects of constant-moderate endurance (END) and high-intensity interval training (HIIT), on heart adiponectin levels in mice.

Material and methods: Two experiments were conducted: (1) preventive (EX1): 10 week-old male mice were fed standard (CHOW) or high-fat diet (HFD;45% fat) and simultaneously trained with END and HIIT for 10 weeks; (2) Treatment (EX2): after 10 weeks of dietary intervention, another cohort of 10 week-old mice were trained by both programmes for 10 weeks.

Results: In EX1, END and HIIT decreased low-molecular weight adiponectin (∼0.5-fold; p < 0.05) and increased GLUT4 levels (∼2-fold; p < .05). In EX2, HFD significantly decreased high-molecular weight adiponectin (∼0.7-fold; p < .05), and END reversed this change.Discussion and conclusion: HFD and exercise influence heart adiponectin isoforms and therefore might impact cardiomyocyte metabolism.

Keywords: GLUT4; Obesity; adiponectin; exercise; heart.

MeSH terms

  • Adiponectin* / metabolism
  • Animals
  • Diet, High-Fat / adverse effects
  • Heart
  • High-Intensity Interval Training*
  • Male
  • Mice
  • Obesity / etiology
  • Obesity / prevention & control


  • Adiponectin