Efficient construction of a stable linear gene based on a TNA loop modified primer pair for gene delivery

Xuehe Lu; Xiaohui Wu; Tiantian Wu; Lin Han; Jianbing Liu; Baoquan Ding

doi:10.1039/d0cc04356g

Efficient construction of a stable linear gene based on a TNA loop modified primer pair for gene delivery

Chem Commun (Camb). 2020 Aug 25;56(68):9894-9897. doi: 10.1039/d0cc04356g.

Authors

Xuehe Lu¹, Xiaohui Wu², Tiantian Wu², Lin Han¹, Jianbing Liu², Baoquan Ding³

Affiliations

¹ School of Materials Science and Engineering, Henan Institute of Advanced Technology, Zhengzhou University, Zhengzhou, 450001, China and CAS Key Laboratory of Nanosystem and Hierarchical Fabrication, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, 100190, China. liujb@nanoctr.cn dingbq@nanoctr.cn.
² CAS Key Laboratory of Nanosystem and Hierarchical Fabrication, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, 100190, China. liujb@nanoctr.cn dingbq@nanoctr.cn and University of Chinese Academy of Sciences, Beijing, 100049, China.
³ School of Materials Science and Engineering, Henan Institute of Advanced Technology, Zhengzhou University, Zhengzhou, 450001, China and CAS Key Laboratory of Nanosystem and Hierarchical Fabrication, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, 100190, China. liujb@nanoctr.cn dingbq@nanoctr.cn and University of Chinese Academy of Sciences, Beijing, 100049, China.

PMID: 32720666
DOI: 10.1039/d0cc04356g

Abstract

A terminal-closed linear gene with strong exonuclease resistance and serum stability was successfully constructed by polymerase chain reaction (PCR) with an α-l-threose nucleic acid (TNA) loop modified primer pair, which can be used as an efficient gene expression system in eukaryotic cells for gene delivery.

MeSH terms

DNA Primers / chemistry*
DNA Primers / metabolism
Gene Expression
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
HEK293 Cells
Humans
Microscopy, Fluorescence
Nucleic Acids / chemistry*
Plasmids / genetics
Plasmids / metabolism
Polymerase Chain Reaction
Tetroses / chemistry*
Transfection / methods*

Substances

DNA Primers
Nucleic Acids
Tetroses
enhanced green fluorescent protein
Green Fluorescent Proteins
erythrose