Nerve growth factor induces a gene homologous to the glucocorticoid receptor gene

Neuron. 1988 May;1(3):183-8. doi: 10.1016/0896-6273(88)90138-9.


Nerve growth factor (NGF) is required for the development and survival of sympathetic and neural crest-derived sensory neurons. The mechanism of action of NGF has been extensively studied in the NGF-responsive rat pheochromocytoma cell line, PC12. When treated with NGF, PC12 cells initiate neurite outgrowth and differentiate into cells with a neuronal phenotype. This process is prevented by RNA synthesis inhibitors. NGFI-B is a gene, identified by differential hybridization, that is rapidly, but transiently induced in PC12 cells by NGF. The nucleotide sequence of the NGFI-B gene was determined, and it encodes a 61 kd protein with strong homologies to members of the glucocorticoid receptor gene family. The two regions of homology between NGFI-B and this family of ligand-dependent transcriptional activators are the region corresponding to the DNA-binding domain and the region comprising the ligand-binding domain near the COOH-terminus. NGFI-B, as a possible ligand-dependent transcriptional activator induced by NGF, may play a role in initiating NGF-induced differentiation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Differentiation / drug effects
  • DNA-Binding Proteins / genetics
  • Gene Expression Regulation*
  • Molecular Sequence Data
  • Nerve Growth Factors / pharmacology*
  • Nuclear Receptor Subfamily 4, Group A, Member 1
  • Pheochromocytoma*
  • Rats
  • Receptors, Cytoplasmic and Nuclear
  • Receptors, Glucocorticoid / genetics*
  • Receptors, Steroid
  • Transcription Factors / genetics
  • Tumor Cells, Cultured / cytology*
  • Tumor Cells, Cultured / drug effects


  • DNA-Binding Proteins
  • Nerve Growth Factors
  • Nr4a1 protein, rat
  • Nuclear Receptor Subfamily 4, Group A, Member 1
  • Receptors, Cytoplasmic and Nuclear
  • Receptors, Glucocorticoid
  • Receptors, Steroid
  • Transcription Factors