Enhanced Effect of Hyaluronan and Elastin Synthesis in Fibroblasts Through Lipopolysaccharide-activated Macrophages

Anticancer Res. 2020 Aug;40(8):4681-4685. doi: 10.21873/anticanres.14467.


Background/aim: The functions of macrophages change in response to environmental factors such as lipopolysaccharide (LPS). LPS derived from Pantoea agglomerans (LPSp) is involved in macrophage activation and tissue repair when administered dermally. LPSp-activated macrophages may be useful for restoring and maintaining homeostasis of the skin.

Materials and methods: Phorbol myristate acetate-treated human monocytes (THP-1 cells) were activated with LPSp. The medium of LPSp-activated THP-1 cells was added to normal human dermal fibroblasts (NHDF cells). After 24 h, the expression of hyaluronan (HA) synthase (HAS)2, hyaluronidase (HYAL)1, and tropoelastin in NHDF cells was analyzed using quantitative real-time PCR.

Results: The expression of HAS2 and tropoelastin was significantly increased, but that of HYAL1 was significantly decreased. It was demonstrated that the abilities of HA and elastin synthesis in NHDF cells increased through LPSp-activated THP-1 cells.

Conclusion: LPSp-activated macrophages may be useful for enhancing the abilities of HA and elastin synthesis in fibroblasts, subsequently improving dysfunction and reducing various age-related disorders.

Keywords: Monocyte; elastin; fibroblast; hyaluronan; lipopolysaccharide; macrophage.

MeSH terms

  • Cell Line
  • Elastin / metabolism*
  • Fibroblasts / drug effects*
  • Fibroblasts / metabolism*
  • Humans
  • Hyaluronic Acid / metabolism*
  • Lipopolysaccharides / pharmacology*
  • Macrophage Activation
  • Macrophages / drug effects*
  • Macrophages / metabolism*
  • Monocytes / drug effects
  • Monocytes / metabolism
  • Pantoea / metabolism
  • Phagocytosis / drug effects
  • Th1 Cells


  • Lipopolysaccharides
  • Hyaluronic Acid
  • Elastin