Mastication Affects Transcriptomes of Mouse Microglia

Anticancer Res. 2020 Aug;40(8):4719-4727. doi: 10.21873/anticanres.14473.


Background/aim: We investigated whether mastication affects microglia, whose activity is thought to be associated with cognition and brain tumor progression.

Materials and methods: We kept mice by feeding either a hard or soft diet for 2, 4 or 8 months. After each period, we removed the whole brains and isolated microglia. The total RNA extracted from each brain's microglia was subjected to DNA microarray analysis.

Results: Many genes were found to be significantly differentially expressed between hard- and soft-diet-fed mice in each group of the same feeding period. The expression of several genes involved in the regulation of actin cytoskeleton was down-regulated in the soft-diet-fed mice.

Conclusion: Mastication may affect microglia's roles in cognition as well as their neuroimmune activity through their activity of patrolling the brain.

Keywords: Brain tumor; DNA microarray; cognition; neuroimmunity; regulation of actin cytoskeleton.

MeSH terms

  • Animals
  • Brain / physiology
  • Male
  • Mastication / physiology*
  • Mice
  • Mice, Inbred C3H
  • Microglia / physiology*
  • Transcriptome / physiology*