Immunomodulatory Effect of a Salvia plebeia R. Aqueous Extract in Forced Swimming Exercise-induced Mice

Nutrients. 2020 Jul 28;12(8):2260. doi: 10.3390/nu12082260.

Abstract

This study investigated the immunomodulatory effect of Salvia plebeia R. aqueous extract (FIE-SP, SPW) in forced swimming exercise-induced mice and the immunostimulatory effects on Raw264.7 cells. Mice were randomly assigned to four groups: the control group (CON), the forced swimming test group (FST), and two FIE-SP groups (low and high dose of FIE-SP). Compared with the control group, the FIE-SP groups showed significantly increased ratios of T lymphocyte surface markers CD4+/CD8+ and major histocompatibility complex (MHC)I/MHCII, as well as increased concentrations of immunoglobulin (Ig)A and IgG. FIE-SP groups significantly increased Th1 cytokines and decreased Th2 cytokines compared with negative control exercise-induced mice. Conversely, the immunostimulatory effects of FIE-SP significantly increased phagocytic activities, nitric oxide (NO) production, and pro-inflammatory cytokines tumor necrosis factor alpha (TNF-α) and interleukin (IL)-1β in Raw264.7 cells. Furthermore, FIE-SP increased natural killer (NK) cell activities and cytokines (IL-12) in splenocytes compared with the CON group. These results indicated that FIE-SP supplementation could prevent imbalanced immune states and produce immunostimulatory effects to support innate immunity.

Keywords: Salvia plebeia R.; adaptive immunity; forced swimming; immunomodulatory effect; innate immunity.

MeSH terms

  • Animals
  • Antigens, Surface / drug effects
  • CD4-CD8 Ratio
  • Cytokines / drug effects
  • Dietary Supplements*
  • Immunity, Innate / drug effects*
  • Immunoglobulin A / drug effects
  • Immunoglobulin G / drug effects
  • Major Histocompatibility Complex / drug effects
  • Mice
  • Plant Extracts / pharmacology*
  • RAW 264.7 Cells
  • Salvia*
  • Swimming

Substances

  • Antigens, Surface
  • Cytokines
  • Immunoglobulin A
  • Immunoglobulin G
  • Plant Extracts