Evaluation of hydrolytic enzyme activities from digestive fluids of Anthonomus grandis (Coleoptera: Curculionidae)

Arch Insect Biochem Physiol. 2020 Oct;105(2):e21730. doi: 10.1002/arch.21730. Epub 2020 Aug 1.

Abstract

The cotton boll weevil, Anthonomus grandis, is a major pest of cotton crops in South America. In this work, partial biochemical characterizations of (hemi) cellulases and pectinases activities in the digestive system (head- and gut- extracts) of A. grandis were evaluated. Gut extract section from third instar larvae exhibited endoglucanase, xylanase, β-glucosidase, and pectinase activities. The endoglucanase and xylanase activities were localized in the foregut, whereas β-glucosidase activity was mainly detected in the hindgut. In addition, no difference in pectinase activity was observed across the gut sections. Thus, A. grandis digestive system is a potentially interesting reservoir for further lignocellulolytic enzymes research.

Keywords: Anthonomus grandis; digestive fluids; enzymatic activities; gut sections; larval instars.

MeSH terms

  • Animals
  • Body Fluids / enzymology
  • Cellulases / chemistry
  • Cellulose / metabolism
  • Digestive System / enzymology*
  • Digestive System / growth & development
  • Head
  • Larva / enzymology
  • Larva / growth & development
  • Polygalacturonase / chemistry
  • Weevils / enzymology*
  • Weevils / growth & development

Substances

  • Cellulose
  • Cellulases
  • Polygalacturonase