Laser-photobiomodulation on experimental cancer pain model in Walker Tumor-256

J Photochem Photobiol B. 2020 Sep:210:111979. doi: 10.1016/j.jphotobiol.2020.111979. Epub 2020 Jul 26.


Context: Cancer Pain is considered a common and significant clinical problem in malignant neoplasms, comprising 20% to 50% of all patients with tumor progression. Laser photobiomodulation (L-PBM) has been used in a multitude of pain events, ranging from acute trauma to chronic articular. However, L-PBM has never been tested in cancer pain.

Objectives: Evaluate hyperalgesia, edema, COX-1, COX-2, IL-10, and Bdkrb1 mRNA in low-level laser irradiated Walker-256 tumor-bearing rats.

Methods: Rat hind paw injected with Walker Tumor-256 (W-256) and divided into six groups of 6 rats: G1 (control) - W-256 injected, G2- W-256 + Nimesulide, G3- W-256 + 1 J, G4- W-256 + 3 Jand G5- W256 + 6 J. Laser parameters: λ = 660 nm, 3.57 W/cm2, Ø = 0.028 cm2. Mechanical hyperalgesia was evaluated by Randall-Selitto test. Plethysmography measured edema; mRNA levels of COX-1, COX-2, IL-10, and Bdkrb1were analyzed.

Results: It was found that the W-256 + 1 J group showed a decrease in paw edema, a significant reduction in pain threshold. Higher levels of IL-10 and lower levels of COX-2 and Bdkrb1 were observed.

Conclusion: Results suggest that 1 J L-PBM reduced the expression of COX-2 and Bdkrb1 and increasing IL-10 gene expression, promoting analgesia to close levels to nimesulide.

Keywords: COX; Cancer pain; IL-10; Inflammation; Laser-photobiomodulation; Low-level laser therapy; Walker Tumor-256.

MeSH terms

  • Animals
  • Carcinoma 256, Walker / metabolism
  • Carcinoma 256, Walker / pathology
  • Cell Line, Tumor
  • Cyclooxygenase 1 / genetics
  • Cyclooxygenase 1 / metabolism
  • Cyclooxygenase 2 / genetics
  • Cyclooxygenase 2 / metabolism
  • Edema / metabolism
  • Edema / pathology
  • Humans
  • Hyperalgesia / radiotherapy*
  • Interleukin-10 / genetics
  • Interleukin-10 / metabolism
  • Lasers, Semiconductor / therapeutic use*
  • Low-Level Light Therapy*
  • Male
  • Plethysmography
  • Rats
  • Rats, Wistar
  • Transplantation, Heterologous


  • Interleukin-10
  • Cyclooxygenase 1
  • Cyclooxygenase 2