Diatoms are found universally in waters around the world. Some diatom species such as Asterionella formosa have a broad variation in seasonal abundance leading to the possibility that diatoms could constrain the time of year when an object was immersed in water. Here we apply this technique to the cold case of DB Cooper's money. Nine years after the crime, six thousand dollars in three bundles were found on the shore of the Columbia River near Portland, Oregon. This burial site was ~30 km from his reported jump location which gave no apparent reason for the money to end up there. This study found diatoms on a recovered bill which indicates that the money was immersed before burial. The species mix found on the bills was compared to a test bill submerged in the Columbia River in November which was the timeframe for the crime. The Cooper bill contained diatoms from summer bloom species suggesting that the money was not directly buried dry and the immersion happened months after the late November hijacking. This finding rules out of a majority of current theories related to the crime and proposes diatoms as a feasible methodology to constrain seasonal timelines in forensics.