Detection of SARS-CoV-2 by RNAscope ® in situ hybridization and immunohistochemistry techniques

Arch Virol. 2020 Oct;165(10):2373-2377. doi: 10.1007/s00705-020-04737-w. Epub 2020 Aug 5.

Abstract

In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Antisense Elements (Genetics) / genetics
  • Betacoronavirus / genetics*
  • Betacoronavirus / isolation & purification*
  • COVID-19
  • COVID-19 Testing
  • Chlorocebus aethiops
  • Clinical Laboratory Techniques / methods*
  • Coronavirus Infections / diagnosis*
  • Coronavirus Infections / virology
  • Coronavirus Nucleocapsid Proteins
  • Genes, Viral
  • Humans
  • Immunohistochemistry / methods
  • In Situ Hybridization / methods
  • Nucleocapsid Proteins / genetics
  • Nucleocapsid Proteins / metabolism
  • Pandemics
  • Phosphoproteins
  • Pneumonia, Viral / diagnosis*
  • Pneumonia, Viral / virology
  • Polyproteins
  • RNA, Viral / genetics*
  • RNA, Viral / metabolism
  • SARS-CoV-2
  • Spike Glycoprotein, Coronavirus / genetics
  • Spike Glycoprotein, Coronavirus / metabolism
  • Vero Cells
  • Viral Proteins / genetics
  • Viral Proteins / metabolism

Substances

  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Antisense Elements (Genetics)
  • Coronavirus Nucleocapsid Proteins
  • Nucleocapsid Proteins
  • ORF1ab polyprotein, SARS-CoV-2
  • Phosphoproteins
  • Polyproteins
  • RNA, Viral
  • Spike Glycoprotein, Coronavirus
  • Viral Proteins
  • nucleocapsid phosphoprotein, SARS-CoV-2
  • spike protein, SARS-CoV-2