Templated insertions at VD and DJ junctions create unique B-cell receptors in the healthy B-cell repertoire

Marvyn T Koning; Esther M Vletter; Rik Rademaker; Rochelle D Vergroesen; Ignis J M Trollmann; Paul Parren; Cornelis A M van Bergen; Hans U Scherer; Szymon M Kiełbasa; René E M Toes; Hendrik Veelken

doi:10.1002/eji.202048828

Templated insertions at VD and DJ junctions create unique B-cell receptors in the healthy B-cell repertoire

Eur J Immunol. 2020 Dec;50(12):2099-2101. doi: 10.1002/eji.202048828. Epub 2020 Aug 27.

Affiliations

¹ Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands.
² Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands.
³ Genmab, Utrecht, The Netherlands.
⁴ Department of Medical Statistics and Bioinformatics, Leiden University Medical Center, Leiden, The Netherlands.

Abstract

RAG complexes recognise (cryptic) RSS sites both in and outside immunoglobulin sites. Excision circles may be reinserted into V(D)J rearrangements as long templated insertions to diversify the adaptive immune repertoire. We show that such VDJ with templated insertions are incidentally found in the repertoire of healthy donors.

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

Adaptive Immunity / genetics
Adaptive Immunity / immunology
B-Lymphocytes / immunology*
Humans
Receptors, Antigen, B-Cell / genetics*
Receptors, Antigen, B-Cell / immunology
V(D)J Recombination / genetics*
V(D)J Recombination / immunology
VDJ Recombinases / genetics*
VDJ Recombinases / immunology

Substances

Receptors, Antigen, B-Cell
VDJ Recombinases