Fabrication and characterization of carboxymethyl guar gum nanocomposite for application of wound healing

Abstract

Bio scaffolds used for cutaneous tissue regeneration is a challenging issue in the healthcare system. To help this problem, we aimed to report on fabrication and characterization of citric acid cross-linked carboxymethyl guar gum (CMGG) nanocomposite films loaded with ciprofloxacin for faster wound healing application. Differential scanning calorimeter (DSC) and attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy studies, dynamic light scattering (DLS), scanning electron microscopy (SEM) and X-ray diffraction (XRD) were used as analytical techniques for characterization of the nanocomposite film. The morphological characters of nanocomposite film were determined by SEM. The prepared scaffolds were evaluated for the carboxyl content and swelling ratio. Ciprofloxacin was loaded into scaffold and drug release was studied at pH 7.4. The hemolysis assay was used to study the biocompatibility of scaffold films. The formation of ester cross-links between citric acid and CMGG was confirmed by DSC and ATR- FTIR. The total carboxyl content of scaffold was found to be decreased when the amount of CMGG was increased. The swelling of scaffold film was found to be decreased with increase in curing temperature and time. CMGG scaffold films showed high drug loading with non-Fickian release mechanism suggesting controlled release of drug. In vivo wound healing studies were carried out for 5 days. In this study we observed a faster wound healing effect within 5 days by incorporation of ciprofloxacin in the CMGG film and found biocompatible. Hence, these Nanocomposite films show greater potential in treating wounds.

Keywords: Biomaterials; Carboxymethyl guar gum; Citric acid; Drug delivery; Nano scaffold; Wound healing.