Design, synthesis and molecular modeling of phenyl dihydropyridazinone derivatives as B-Raf inhibitors with anticancer activity

Abstract

Three new series of phenyl dihydropyridazinone derivatives 4b-8i have been designed, synthesized and evaluated for their anticancer activity against different cancer cell lines. Nine compounds showed strong inhibitory activity, among which compound 8b exhibited potent activity against PC-3 cell line with IC₅₀ value of 7.83 µM in comparison to sorafenib (IC₅₀ 11.53 µM). Compounds 6a, 6c, 7f-h and 8a-d were further screened for their B-Raf inhibitory activity where seven compounds 7f-h and 8a-d showed high B-Raf inhibition with ranges of IC₅₀ values 70.65-84.14 nM and 24.97-44.60 nM, respectively when compared to sorafenib (IC₅₀ 44.05 nM). Among the tested compounds, 8b was the most potent B-Raf inhibitor with IC₅₀ value of 24.79 nM. Cell cycle analysis of MCF-7 cells treated with 8b showed cell cycle arrest at G2-M phase with significant apoptotic effect. Molecular modeling study was performed to understand the binding mode of the most active synthesized compounds with B-Raf enzyme.

Keywords: Antitumor; B-Raf; Cell cycle analysis; Molecular modeling; Pyridazinone.