A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions

G3 (Bethesda). 2020 Sep 2;10(9):3399-3402. doi: 10.1534/g3.120.401554.


The world is facing a global pandemic of COVID-19 caused by the SARS-CoV-2 coronavirus. Here we describe a collection of codon-optimized coding sequences for SARS-CoV-2 cloned into Gateway-compatible entry vectors, which enable rapid transfer into a variety of expression and tagging vectors. The collection is freely available. We hope that widespread availability of this SARS-CoV-2 resource will enable many subsequent molecular studies to better understand the viral life cycle and how to block it.

Keywords: Gateway-compatible; SARS-CoV-2; TEV (tobacco etch virus) sequence; coding sequence collection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Betacoronavirus / genetics*
  • Betacoronavirus / isolation & purification
  • COVID-19
  • Cloning, Molecular
  • Coronavirus Infections / pathology
  • Coronavirus Infections / virology
  • Escherichia coli / metabolism
  • Humans
  • Open Reading Frames / genetics*
  • Pandemics
  • Plasmids / genetics
  • Plasmids / metabolism
  • Pneumonia, Viral / pathology
  • Pneumonia, Viral / virology
  • Potyvirus / genetics
  • SARS-CoV-2

Supplementary concepts

  • Tobacco etch virus

Associated data

  • figshare/10.25387/g3.12725096