Arbuscular mycorrhizal fungi (AMF) can form symbiosis with Elaeagnus angustifolia, allowing this species to tolerate salt stress. However, the physiological mechanism through which AMF improve E. angustifolia tolerance is still unclear. In this study, we examined E. angustifolia inoculated with AMF Rhizophagus irregularis (M) or inactivated inoculum (NM) under 0 and 300 mM NaCl stress for the determination of photosynthetic gas exchange, pigment content, chlorophyll fluorescence, antioxidant capacity and chloroplast ultrastructural in leaves. Photosynthetic gas exchange parameters in the leaves of M and NM decreased significantly under salt stress, while the M treatment significantly reduced the effect of salt stress compared with NM. Various chlorophyll components in the M treatment were two- to three-fold higher than in NM, together with a much more complex chloroplast structure and higher number of plastoglobules. The total flavonoid and proline content in leaves of M increased significantly, while the concentration of malondialdehyde (MDA) decreased significantly under salt stress. Chlorophyll fluorescence data also showed good PSII function in the M treatment, together with salt stress reduction of photochemical reactions and sharp enhancements in non-photosynthetic quenching (NPQ). AMF inoculation ameliorated the inhibition on the actual PSII efficiency (ФPSII) and the photochemical quenching coefficient (qP ) by 10-15%. Our results clearly demonstrate that R. irregularis can improve the salt tolerance of plants by improving leaf photosynthetic performance, PSII function, antioxidant capacity and leaf chloroplast ultrastructure, and that E. angustifolia inoculated with AMF could enhance saline soil rehabilitation.
Keywords: Rhizophagus irregularis; oleaster; photosynthetic parameters; photosystem II; salt stress.
© 2020 German Society for Plant Sciences and The Royal Botanical Society of the Netherlands.