Huoxuezhitong capsule ameliorates MIA-induced osteoarthritis of rats through suppressing PI3K/ Akt/ NF-κB pathway

Biomed Pharmacother. 2020 Sep;129:110471. doi: 10.1016/j.biopha.2020.110471. Epub 2020 Jul 4.

Abstract

Huoxuezhitong capsule (HXZT, activating blood circulation and relieving pain capsule), has been applied for osteoarthritis since 1974. It consists of Angelica sinensis (Oliv.) Diels, Panax notoginseng (Burkill) F. H. Chen ex C. H., Boswellia sacra, Borneol, Eupolyphaga sinensis Walker, Pyritum. However, the direct effects of HXZT on osteoarthritis and the underlying mechanisms were poorly understood. In this study, we aimed to explore the analgesia effect of HXZT on MIA-induced osteoarthritis rat and the underlying mechanisms. The analgesia and anti-inflammatory effect of HXZT on osteoarthritis in vivo were tested by the arthritis model rats induced by monosodium iodoacetate (MIA).. Mechanistic studies confirmed that HXZT could inhibit the activation of NF-κB and down-regulate the mRNA expression of related inflammatory factors in LPS-induced RAW264.7 and ATDC5 cells. Furtherly, in LPS-induced RAW264.7 cells, HXZT could suppress NF-κB via inhibiting PI3K/Akt pathway. Taken together, HXZT capsule could ameliorate MIA-induced osteoarthritis of rats through suppressing PI3K/ Akt/ NF-κB pathway.

Keywords: Analgesia; Anti-inflammation; Ginsenoside Rg1 (CID: 441923); Huoxuezhitong capsule; Lipopolysaccharide (LPS) (CID: 11970143); Notoginsenoside R1 (CID: 441934); Osteoarthritis; PI3K/Akt/ NF-κB pathway; Sodium iodoacetate (MIA) (CID: 5239).

MeSH terms

  • Animals
  • Antirheumatic Agents / pharmacology*
  • Arthritis, Experimental / chemically induced
  • Arthritis, Experimental / enzymology
  • Arthritis, Experimental / pathology
  • Arthritis, Experimental / prevention & control*
  • Capsules
  • Cytokines / metabolism
  • Drugs, Chinese Herbal / pharmacology*
  • Inflammation Mediators / metabolism
  • Iodoacetic Acid
  • Knee Joint / drug effects*
  • Knee Joint / enzymology
  • Knee Joint / pathology
  • Male
  • Mice
  • NF-kappa B / metabolism*
  • Osteoarthritis, Knee / chemically induced
  • Osteoarthritis, Knee / enzymology
  • Osteoarthritis, Knee / pathology
  • Osteoarthritis, Knee / prevention & control*
  • Phosphatidylinositol 3-Kinase / metabolism*
  • Phosphorylation
  • Proto-Oncogene Proteins c-akt / metabolism*
  • RAW 264.7 Cells
  • Rats, Sprague-Dawley
  • Signal Transduction

Substances

  • Antirheumatic Agents
  • Capsules
  • Cytokines
  • Drugs, Chinese Herbal
  • Inflammation Mediators
  • NF-kappa B
  • huoxuezhitong capsule
  • Phosphatidylinositol 3-Kinase
  • Proto-Oncogene Proteins c-akt
  • Iodoacetic Acid