MicroRNA Profiling in Paired Left and Right Eyes, Lungs, and Testes of Normal Mice

Jiangcheng Shi; Chengqing Hu; Yuan Zhou; Chunmei Cui; Jichun Yang; Qinghua Cui

doi:10.1016/j.omtn.2020.07.006

MicroRNA Profiling in Paired Left and Right Eyes, Lungs, and Testes of Normal Mice

Mol Ther Nucleic Acids. 2020 Sep 4:21:687-695. doi: 10.1016/j.omtn.2020.07.006. Epub 2020 Jul 10.

Authors

Jiangcheng Shi¹, Chengqing Hu¹, Yuan Zhou¹, Chunmei Cui¹, Jichun Yang², Qinghua Cui³

Affiliations

¹ Department of Biomedical Informatics, Department of Physiology and Pathophysiology, Center for Noncoding RNA Medicine, MOE Key Lab of Cardiovascular Sciences, School of Basic Medical Sciences, Peking University, 38 Xueyuan Rd., Beijing 100191, China.
² Department of Biomedical Informatics, Department of Physiology and Pathophysiology, Center for Noncoding RNA Medicine, MOE Key Lab of Cardiovascular Sciences, School of Basic Medical Sciences, Peking University, 38 Xueyuan Rd., Beijing 100191, China. Electronic address: yangj@bjmu.edu.cn.
³ Department of Biomedical Informatics, Department of Physiology and Pathophysiology, Center for Noncoding RNA Medicine, MOE Key Lab of Cardiovascular Sciences, School of Basic Medical Sciences, Peking University, 38 Xueyuan Rd., Beijing 100191, China. Electronic address: cuiqinghua@bjmu.edu.cn.

Abstract

Physiological and pathophysiological differences widely exist in paired organ systems. However, the molecular basis for these differences remains largely unknown. We previously reported that there exist differentially expressed miRNAs (DEMs) in the left and right kidneys of normal mice. Here, we identified the DEMs in the left and right eyes, lungs, and testes of normal mice via RNA sequencing. As a result, we identified 26 DEMs in eyes, with 23 higher and 3 lower in the left eyes compared with right eyes; 21 DEMs in lungs, with 15 higher and 6 lower in the left lungs compared with right lungs; and 54 DEMs in testes, with 6 higher and 48 lower in the left testes compared with right testes. Ten microRNAs (miRNAs) were further examined by quantitative PCR assays, and seven of these were confirmed. In addition, correlation analysis was performed between paired organ miRNA expressions and diverse body fluid miRNA expressions. Finally, we explored the functions and networks of DEMs and performed biological process and pathway enrichment analysis of target genes for DEMs, providing insights into the physiological and pathophysiological differences between the two entities of paired organs.

Keywords: bioinformatic analysis; gene profile; miRNA; paired organ.