ICAM-1 a ligand for LFA-1-dependent adhesion of B, T and myeloid cells

Nature. 1988 Jan 7;331(6151):86-8. doi: 10.1038/331086a0.


Cell-cell adhesion is essential for many immunological functions. The LFA-1 molecule, a member of a superfamily of adhesion molecules, participates in adhesion which is critical to the function of each of the three major subsets of leukocytes: lymphocytes, monocytes and granulocytes. Putative LFA-1 ligands have been identified functionally in different laboratories using three different monoclonal antibodies that inhibit LFA-1-mediated leukocyte adhesion in particular model systems; however, there may be more than one LFA-1 ligand. We have directly compared the three relevant monoclonal antibodies, and show that each binds to the same molecule, intercellular-adhesion molecule-1 (ICAM-1). Most important, B, T and myeloid cells adhere specifically to purified ICAM-1-coated surfaces; such adhesion has distinctive requirements for Mg2+ and Ca2+. This constitutes biochemical evidence that ICAM-1 functions as a ligand for LFA-1-dependent adhesion by a variety of leukocytes.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Antigens, Surface / immunology
  • Antigens, Surface / physiology*
  • B-Lymphocytes / physiology*
  • Calcium / physiology
  • Cell Adhesion*
  • Hematopoietic Stem Cells / physiology*
  • Lymphocyte Function-Associated Antigen-1
  • Magnesium / physiology
  • T-Lymphocytes, Cytotoxic / physiology*
  • Tumor Cells, Cultured


  • Antibodies, Monoclonal
  • Antigens, Surface
  • Lymphocyte Function-Associated Antigen-1
  • Magnesium
  • Calcium