Mechanism of autonomous control of the Escherichia coli F plasmid: purification and characterization of the repE gene product

Nucleic Acids Res. 1988 Jan 25;16(2):413-24. doi: 10.1093/nar/16.2.413.

Abstract

E protein, the 29 Kd repE gene product, is essential for the replication of the Escherichia coli F plasmid. The repE gene has been cloned and expressed in an inducible ATG-fusion vector, and the protein product has been purified to homogeneity. The purified E protein is present as a dimer in solution and binds specifically to both the 19-bp direct repeats (incB) found within the mini-F origin of replication ori2 and the repE operator DNA. Examination of the amino acid sequence of E protein revealed a consensus sequence for DNA binding.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / isolation & purification
  • DNA Replication*
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / isolation & purification
  • Escherichia coli / genetics*
  • F Factor*
  • Kinetics
  • Operator Regions, Genetic

Substances

  • Bacterial Proteins
  • DNA-Binding Proteins