Time-resolved cryo-EM using Spotiton

Nat Methods. 2020 Sep;17(9):897-900. doi: 10.1038/s41592-020-0925-6. Epub 2020 Aug 10.


We present an approach for preparing cryo-electron microscopy (cryo-EM) grids to study short-lived molecular states. Using piezoelectric dispensing, two independent streams of ~50-pl droplets of sample are deposited within 10 ms of each other onto the surface of a nanowire EM grid, and the mixing reaction stops when the grid is vitrified in liquid ethane ~100 ms later. We demonstrate this approach for four biological systems where short-lived states are of high interest.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cryoelectron Microscopy / methods*
  • Nanowires
  • Robotics
  • Specimen Handling / methods
  • Time Factors