Algibacter marinivivus sp. nov., isolated from the surface of a marine red alga

Abstract

A Gram-stain-negative, non-flagellated bacterium, designated ZY111^T, was isolated from the surface of a marine red alga, which was collected from the coast in Weihai, Shandong Province, PR China. Strain ZY111^T exhibited growth at 4-37 °C (optimum, 25-28 °C) in the presence of 0-8.0 % (w/v) NaCl (optimum, 2.0-4.0% NaCl) and at pH 6.5-9.5 (optimum, pH 7.0-8.0). The 16S rRNA gene sequence analysis revealed that strain ZY111^T belonged to the genus Algibacter, with Algibacter amylolyticus DSM 29199^T as its closest relative (97.7 % similarity). The averagenucleotide identity value of strain ZY111^T with A. amylolyticus DSM 29199^T was 79.03 %. The digitalDNA-DNA hybridization value of strain ZY111^T with A. amylolyticus DSM 29199^T was 22.40 %. The dominant fatty acids were iso-C_{15 : 0}, iso-C_{15 : 1} G, iso-C_{15 : 0} 3-OH and iso-C_{17 : 0} 3-OH. The sole respiratory quinone was determined to be menaquinone-6. The polar lipid profile of strain ZY111^T consisted of phosphatidylethanolamine, two unidentified aminolipids and three unidentified lipids. The G+C content was 31.9 mol%. The phenotypic, chemotaxonomic and phylogenetic data clearly showed that strain ZY111^T represents a novel species of the genus Algibacter, for which the name Algibacter marinivivus sp. nov. is proposed. The type strain is ZY111^T (=KCTC 62373^T=MCCC 1H00295^T).

Keywords: 16SrRNA gene; Algibacter; phylogenetic analysis.