Abstract
Here we propose a general strategy to label carbohydrates with N-methyl-anthranilic acid at the anomeric position. Through two examples, we demonstrate that the generated glycoprobes are suitable for fluorescence-based binding/competition assays. Our approach is expected to readily generate series of glycoprobes dedicated to screening assays for the discovery of drugs targeting carbohydrate-protein interactions.
MeSH terms
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Fluorescent Dyes / chemical synthesis
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Fluorescent Dyes / chemistry*
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Fluorescent Dyes / metabolism
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Glycosides / chemical synthesis
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Glycosides / chemistry*
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Glycosides / metabolism
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Maltose-Binding Proteins / metabolism
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Protein Binding
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Spectrometry, Fluorescence
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ortho-Aminobenzoates / chemical synthesis
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ortho-Aminobenzoates / chemistry*
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ortho-Aminobenzoates / metabolism
Substances
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Fluorescent Dyes
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Glycosides
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Maltose-Binding Proteins
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ortho-Aminobenzoates