Effects of occlusal disharmony on susceptibility to atrial fibrillation in mice

Sci Rep. 2020 Aug 13;10(1):13765. doi: 10.1038/s41598-020-70791-8.

Abstract

Tooth loss or incorrect positioning causes occlusal disharmony. Furthermore, tooth loss and atrial fibrillation (AF) are both risk factors for ischemic stroke and coronary heart disease. Therefore, we hypothesized that occlusal disharmony-induced stress increases susceptibility to AF, and we designed the present study to test this idea in mice. Bite-opening (BO) was done by cementing a suitable appliance onto the mandibular incisor to cause occlusal disharmony by increasing the vertical height of occlusion by 0.7 mm for a period of 2 weeks. AF susceptibility, evaluated in terms of the duration of AF induced by transesophageal burst pacing, was significantly increased concomitantly with atrial remodeling, including fibrosis, myocyte apoptosis and oxidative DNA damage, in BO mice. The BO-induced atrial remodeling was associated with increased calmodulin kinase II-mediated ryanodine receptor 2 phosphorylation on serine 2814, as well as inhibition of Akt phosphorylation. However, co-treatment with propranolol, a non-selective β-blocker, ameliorated these changes in BO mice. These data suggest that improvement of occlusal disharmony by means of orthodontic treatment might be helpful in the treatment or prevention of AF.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenergic beta-Antagonists / therapeutic use
  • Animals
  • Apoptosis / physiology
  • Atrial Fibrillation / pathology*
  • Atrial Fibrillation / prevention & control*
  • Atrial Remodeling / physiology*
  • Calcium-Calmodulin-Dependent Protein Kinases / metabolism
  • Coronary Disease / etiology
  • Coronary Disease / pathology
  • Disease Susceptibility
  • Fibrosis / pathology
  • Ischemic Stroke / etiology
  • Ischemic Stroke / pathology
  • Male
  • Malocclusion / pathology*
  • Malocclusion / therapy*
  • Mice
  • Mice, Inbred C57BL
  • Muscle Cells / pathology
  • Orthodontics / methods*
  • Oxidative Stress / genetics
  • Phosphorylation
  • Propranolol / therapeutic use
  • Proto-Oncogene Proteins c-akt / metabolism
  • Ryanodine Receptor Calcium Release Channel / metabolism

Substances

  • Adrenergic beta-Antagonists
  • Ryanodine Receptor Calcium Release Channel
  • ryanodine receptor 2. mouse
  • Propranolol
  • Proto-Oncogene Proteins c-akt
  • Calcium-Calmodulin-Dependent Protein Kinases