TRPA1 mediates damage of the retina induced by ischemia and reperfusion in mice

Cell Death Dis. 2020 Aug 15;11(8):633. doi: 10.1038/s41419-020-02863-6.

Abstract

Oxidative stress is implicated in retinal cell injury associated with glaucoma and other retinal diseases. However, the mechanism by which oxidative stress leads to retinal damage is not completely understood. Transient receptor potential ankyrin 1 (TRPA1) is a redox-sensitive channel that, by amplifying the oxidative stress signal, promotes inflammation and tissue injury. Here, we investigated the role of TRPA1 in retinal damage evoked by ischemia (1 hour) and reperfusion (I/R) in mice. In wild-type mice, retinal cell numbers and thickness were reduced at both day-2 and day-7 after I/R. By contrast, mice with genetic deletion of TRPA1 were protected from the damage seen in their wild-type littermates. Daily instillation of eye drops containing two different TRPA1 antagonists, an oxidative stress scavenger, or a NADPH oxidase-1 inhibitor also protected the retinas of C57BL/6J mice exposed to I/R. Mice with genetic deletion of the proinflammatory TRP channels, vanilloid 1 (TRPV1) or vanilloid 4 (TRPV4), were not protected from I/R damage. Surprisingly, genetic deletion or pharmacological blockade of TRPA1 also attenuated the increase in the number of infiltrating macrophages and in the levels of the oxidative stress biomarker, 4-hydroxynonenal, and of the apoptosis biomarker, active caspase-3, evoked by I/R. These findings suggest that TRPA1 mediates the oxidative stress burden and inflammation that result in murine retinal cell death. We also found that TRPA1 (both mRNA and protein) is expressed by human retinal cells. Thus, it is possible that inhibition of a TRPA1-dependent pathway could also attenuate glaucoma-related retinal damage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Death
  • Inflammation
  • Ischemia
  • Male
  • Mice
  • Mice, Inbred C57BL
  • NADPH Oxidase 1 / metabolism
  • Oxidative Stress / physiology
  • Reperfusion
  • Reperfusion Injury / metabolism*
  • Reperfusion Injury / physiopathology
  • Retina / metabolism*
  • Retina / physiology
  • Retinal Diseases
  • TRPA1 Cation Channel / genetics
  • TRPA1 Cation Channel / metabolism*
  • TRPA1 Cation Channel / physiology
  • TRPV Cation Channels / metabolism
  • Transient Receptor Potential Channels / genetics

Substances

  • TRPA1 Cation Channel
  • TRPA1 protein, human
  • TRPV Cation Channels
  • TRPV1 protein, mouse
  • Transient Receptor Potential Channels
  • Trpa1 protein, mouse
  • Trpv4 protein, mouse
  • NADPH Oxidase 1