Antifatigue Activity of Glycoprotein from Schisandra chinensis Functions by Reducing Oxidative Stress

Shuai Shao; Ming-Xing Wang; Hong-Yin Zhang; Lin Fan; Rong-Xin Han; Ying-Xin Shen; Ming-Ming Yan; Da-Qing Zhao

doi:10.1155/2020/4231340

Antifatigue Activity of Glycoprotein from Schisandra chinensis Functions by Reducing Oxidative Stress

Evid Based Complement Alternat Med. 2020 Jul 29:2020:4231340. doi: 10.1155/2020/4231340. eCollection 2020.

Authors

Shuai Shao^{1

2}, Ming-Xing Wang³, Hong-Yin Zhang^{1

2}, Lin Fan^{1

2}, Rong-Xin Han^{1

2}, Ying-Xin Shen^{1

2}, Ming-Ming Yan^{1

2}, Da-Qing Zhao^{1

2}

Affiliations

¹ Changchun University of Chinese Medicine, Changchun, Jilin 130117, China.
² Jilin Provincial Science and Technology Innovation Center of Health Food of Chinese Medicine, Changchun University of Chinese Medicine, Changchun, Jilin, China.
³ Research Center of Traditional Chinese Medicine, Affiliated Hospital, Changchun University of Chinese Medicine, Changchun 130021, China.

Abstract

The glycoprotein from Schisandra chinensis was obtained with alkali extraction and acid precipitation, purified with DEAE Sepharose Fast Flow and Superdex G-75 column. The molecular composition structure and antifatigue activities of glycoprotein were studied. SCGP's molecular weight was approximately 10 KDa, and it consisted of a carbohydrate component (52.94%) and protein component (47.06%). SCGP comprised mannose, galactoside, rhamnose, glucose, galactose, xylose, arabinose, and fucose, its molar ratio was 2.14 : 1.43 : 1.59 : 8.17 : 8.99 : 3.18 : 18.51 : 1, and it contained 16 kinds of amino acids. SCGP could obviously extend the swimming time in mice by increasing LDH, SOD level, GSH-Px activity, and liver glycogen and decreasing the contents of BUN and MDA. The antioxidant activity of SCGP is a potential mechanism of its antifatigue effect. In vitro antioxidant test showed that SCGP scavenged DPPH and OH radicals in a dose-dependent manner (IC₅₀ was 0.91 mg/ml and 0.72 mg/ml).