New Phage cocktail against infantile Sepsis bacteria

Abstract

A total of 54 positive blood aerobic cultures of suspected blood sepsis in neonates were purified, diagnosed and tested for antibiotic susceptibility. Six bacterial pathogens of Escherichia coli (10 isolates), Klebsiella pneumoniae (10 isolates), Haemophilus influenzae (4 isolates), Pseudomonas aeruginosa (3 isolates), Citrobacter fruendii (1 isolate) and Moraxella catarrhalis (1 isolate) were selected for preparation of phages active against all isolates of each species. Virulent phages towards bacterial isolates were isolated from sewage water by spot lysis method, and a total of 29 phages active towards selected bacterial pathogens were isolated and purified. Phage(s) active against each bacterial species showed activity spectrum within each species of 30-80%, 50-80%, 50%, 50%, 100% and 100% for isolates of Escherichia coli, Klebsiella spp., Haemophilus influenzae, Pseudomonas aeruginosa, Citrobacter fruendii and Moraxella catarrhalis, respectively. A total of 29 phages were formulated in one cocktail in a concentration of 10⁶ PFU/ml in SM buffer for each of a total of 29 phages in SM buffer and showed activity spectrum of 100% against all their bacterial hosts in vitro.

Keywords: Bacteriophgaes; Neonatal sepsis; Phage therapy.