Genetic alterations of the c-erbB-2 oncogene occur frequently in tubular adenocarcinoma of the stomach and are often accompanied by amplification of the v-erbA homologue

Oncogene. 1988 Mar;2(3):283-7.


We analyzed for alterations of the c-erbB-2 oncogene in 35 human stomach cancers and 8 cell lines derived from human stomach cancer. Amplification of c-erbB-2 was found in approximately 40% (5/13) of the tubular adenocarcinomas of the stomach examined, including 4 of 10 fresh tumors and one of 3 cell lines, but not in other histological types of stomach cancer examined (0/30), including 25 fresh tumors and 5 cell lines. This result strongly suggests that amplification of c-erbB-2 occurs frequently in tubular carcinomas in stomach cancer. Rearrangement of c-erbB-2 was also detected in one tubular adenocarcinoma. The rearranged fragment carried the 3' half, but not the 5' sequence, of the c-erbB-2 gene. Furthermore, one of the cellular homologues of v-erbA was amplified in 3 of 4 fresh tumors carrying the amplified c-erbB-2 gene. Both c-erbB-2 and the v-erbA homologue were expressed in all the stomach cancer cell lines tested.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / genetics*
  • Cell Line
  • Chromosomes, Human, Pair 17
  • Chromosomes, Human, Pair 3
  • DNA, Neoplasm / genetics
  • Gene Amplification
  • Humans
  • Oncogenes*
  • Proto-Oncogene Proteins / genetics*
  • RNA, Messenger / genetics
  • RNA, Neoplasm / genetics
  • Stomach Neoplasms / genetics*


  • DNA, Neoplasm
  • Proto-Oncogene Proteins
  • RNA, Messenger
  • RNA, Neoplasm