Mitochondrial electron transport chain: Oxidative phosphorylation, oxidant production, and methods of measurement

Redox Biol. 2020 Oct;37:101674. doi: 10.1016/j.redox.2020.101674. Epub 2020 Aug 6.


The mitochondrial electron transport chain utilizes a series of electron transfer reactions to generate cellular ATP through oxidative phosphorylation. A consequence of electron transfer is the generation of reactive oxygen species (ROS), which contributes to both homeostatic signaling as well as oxidative stress during pathology. In this graphical review we provide an overview of oxidative phosphorylation and its inter-relationship with ROS production by the electron transport chain. We also outline traditional and novel translational methodology for assessing mitochondrial energetics in health and disease.

Keywords: Electron transport chain; Mitochondria; Mitochondrial reactive oxygen species; Oxidative phosphorylation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Electron Transport
  • Mitochondrial Membranes*
  • Oxidants*
  • Oxidative Phosphorylation*
  • Oxidative Stress
  • Reactive Oxygen Species


  • Oxidants
  • Reactive Oxygen Species