This study aimed to investigate the blood supply of early lung adenocarcinomas in mice and the relationship between tumors and their supplying vessels by using micro-CT. An early lung adenocarcinoma model was established in 10 female mice with subcutaneous injections of a 1-methyl-3-nitro-1-nitrosoguanidine solution. Micro-CT pulmonary and bronchial arteriography were performed to demonstrate the blood supply of early lung adenocarcinomas, especially the tumor-vessel relationships, and the findings were correlated with the pathology results. The quantitative and texture changes in the tumor-supplying vessels were analyzed. Micro-CT showed that the pulmonary artery was densely distributed in and around tumors in 141 (84%) of 167 early lung adenocarcinomas, the bronchial artery was not related to tumors, and there were four patterns of tumor-pulmonary artery relationships that correlated well with pathologic findings. Quantitative and texture analyses showed that the tumor size had positive correlations with vessel volume (VV), VV fraction (VVF), vessel thickness (VT), vessel number (VN), inverse difference moment, long run emphasis, gray level nonuniformity (GLN), and run length nonuniformity (RLN) and negative correlations with vessel separation (VS), inertia, and short run emphasis (SRE); the size of the solid component had positive correlations with VV, VVF, VT, VN, GLN, and RLN and negative correlations with VS, cluster shade, and SRE. This study concluded that early lung adenocarcinomas are mainly supplied by the pulmonary arteries in mice, and micro-CT angiography can clearly demonstrate the morphologic changes of pulmonary arteries and their relationships with tumors.
©2020 American Association for Cancer Research.