70K heat shock related proteins stimulate protein translocation into microsomes

Nature. 1988 Apr 28;332(6167):805-10. doi: 10.1038/332805a0.

Abstract

A yeast cytosol is shown to contain two distinct activities that stimulate protein translocation across microsomal membranes. One activity was purified. It consists of two constitutively expressed 70K heat shock related proteins that increase the rate of translocation. Possible mechanisms of action of these proteins are discussed.

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Affinity Labels / metabolism
  • Biological Transport, Active / drug effects
  • Dithiothreitol / pharmacology
  • Electrophoresis, Polyacrylamide Gel
  • Ethylmaleimide / pharmacology
  • Heat-Shock Proteins / pharmacology*
  • Intracellular Membranes / drug effects*
  • Microsomes / drug effects*
  • Microsomes / metabolism
  • Photochemistry
  • Protein Denaturation
  • Proteins / metabolism*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae / ultrastructure
  • Urea

Substances

  • Affinity Labels
  • Heat-Shock Proteins
  • Proteins
  • Adenosine Triphosphate
  • Urea
  • Ethylmaleimide
  • Dithiothreitol