Genome-Wide Mapping of DNA Methylation 5mC by Methylated DNA Immunoprecipitation (MeDIP)-Sequencing

Methods Mol Biol. 2021:2198:301-310. doi: 10.1007/978-1-0716-0876-0_23.

Abstract

Methylated DNA immunoprecipitation is a large scale purification technique. It enables the isolation of methylated DNA fragments for subsequent locus-specific or genome-wide analysis. Here we describe an immunoprecipitation protocol using a monoclonal mouse anti 5-methyl-cytidine antibody followed by next-generation sequencing (MeDIP-Seq).

Keywords: DNA methylation; Epigenetics; Immuno-precipitation; Methylated DNA immunoprecipitation; Next generation sequencing; Review.

MeSH terms

  • Animals
  • Base Sequence
  • Chromosome Mapping
  • Cytidine / analogs & derivatives*
  • Cytidine / immunology
  • DNA / genetics
  • DNA / immunology
  • DNA Methylation / genetics
  • DNA Methylation / immunology
  • Genome
  • Genome-Wide Association Study / methods
  • High-Throughput Nucleotide Sequencing / methods
  • Humans
  • Immunoprecipitation / methods*
  • Sequence Analysis, DNA / methods*

Substances

  • Cytidine
  • DNA
  • 5-methylcytidine