Recent advances in analytical methods for the determination of citrinin in food matrices

J Chromatogr A. 2020 Sep 13;1627:461399. doi: 10.1016/j.chroma.2020.461399. Epub 2020 Jul 9.


Citrinin is a toxic small organic molecule produced as a secondary metabolite by fungi types Penicillium, Monascus and Aspergillus and is known to contaminate various food commodities during postharvest stages of food production. During the last 10 years, most reported methods for citrinin analysis employed enzyme-linked immunosorbent assays or high-performance liquid chromatography. Over this same time period, liquid extraction, solid-phase extraction, dispersive liquid-liquid microextraction and QuEChERS were the most cited sample preparation and clean-up methods. In this review the advantages and disadvantages of the various sample preparation, separation and detection methods for citrinin analysis over the last decade are evaluated. Furthermore, current trends, emerging technologies and the future prospects of these methods are discussed.

Keywords: Citrinin; Dihydrocitrinone; Extraction; Liquid chromatography; Mass spectrometry; Mycotoxin analysis; Sample preparation.

MeSH terms

  • Aspergillus / metabolism
  • Chromatography, High Pressure Liquid / methods*
  • Citrinin / analysis*
  • Citrinin / isolation & purification
  • Citrinin / urine
  • Enzyme-Linked Immunosorbent Assay
  • Food Contamination / analysis
  • Humans
  • Liquid Phase Microextraction
  • Monascus / metabolism
  • Solid Phase Extraction
  • Tandem Mass Spectrometry / methods*


  • Citrinin