Abstract
Electron microscopic double-label immunostaining with peroxidase and avidin-ferritin was used to study connections between corticotrophin-releasing factor (CRF) and gonadotrophin-releasing hormone (GnRH) immunoreactive elements in the medial preoptic area of the rat. Synaptic contacts were observed between CRF-immunoreactive axon terminals and the dendrites of GnRH-immunopositive neurons. These results suggest that the inhibitory effects of stress-induced CRF release on reproductive function may involve a direct CRF input to the GnRH-producing cells.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Animals
-
Corticotropin-Releasing Hormone / analysis*
-
Corticotropin-Releasing Hormone / immunology
-
Female
-
Immunoenzyme Techniques
-
Microscopy, Electron
-
Neurons / cytology*
-
Neurons / ultrastructure
-
Pituitary Hormone-Releasing Hormones / analysis*
-
Pituitary Hormone-Releasing Hormones / immunology
-
Preoptic Area / cytology*
-
Rats
-
Rats, Inbred Strains
-
Synapses / cytology*
-
Synapses / ultrastructure
Substances
-
Pituitary Hormone-Releasing Hormones
-
Corticotropin-Releasing Hormone