Acidic electrolyzed water potently inactivates SARS-CoV-2 depending on the amount of free available chlorine contacting with the virus

Biochem Biophys Res Commun. 2020 Sep 10;530(1):1-3. doi: 10.1016/j.bbrc.2020.07.029. Epub 2020 Jul 14.


Alcohol-based disinfectant shortage is a serious concern in the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. Acidic electrolyzed water (EW) with a high concentration of free available chlorine (FAC) shows strong antimicrobial activity against bacteria, fungi, and viruses. Here, we assessed the SARS-CoV-2-inactivating efficacy of acidic EW for use as an alternative disinfectant. The quick virucidal effect of acidic EW depended on the concentrations of contained-FAC. The effect completely disappeared in acidic EW in which FAC was lost owing to long-time storage after generation. In addition, the virucidal activity increased proportionately with the volume of acidic EW mixed with the virus solution when the FAC concentration in EW was same. These findings suggest that the virucidal activity of acidic EW against SARS-CoV-2 depends on the amount of FAC contacting the virus.

Keywords: Acidic electrolyzed water; Disinfectant; Free available chlorine; SARS-CoV-2.

MeSH terms

  • Acids / chemistry
  • Acids / pharmacology
  • Antiviral Agents / chemistry
  • Antiviral Agents / pharmacology
  • Betacoronavirus / drug effects*
  • Betacoronavirus / physiology
  • COVID-19
  • Chlorine / chemistry
  • Chlorine / pharmacology*
  • Coronavirus Infections / prevention & control
  • Coronavirus Infections / virology
  • Disinfectants / chemistry
  • Disinfectants / pharmacology*
  • Disinfection / methods*
  • Electrolysis / methods
  • Humans
  • Pandemics / prevention & control
  • Pneumonia, Viral / prevention & control
  • Pneumonia, Viral / virology
  • SARS-CoV-2
  • Virus Inactivation / drug effects*
  • Water / chemistry
  • Water / pharmacology


  • Acids
  • Antiviral Agents
  • Disinfectants
  • Water
  • Chlorine