Virus-Based CRISPR/Cas9 Genome Editing in Plants

Huawei Liu; Baohong Zhang

doi:10.1016/j.tig.2020.08.002

Virus-Based CRISPR/Cas9 Genome Editing in Plants

Trends Genet. 2020 Nov;36(11):810-813. doi: 10.1016/j.tig.2020.08.002. Epub 2020 Aug 19.

Authors

Huawei Liu¹, Baohong Zhang²

Affiliations

¹ College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, PR China. Electronic address: bioche@nwafu.edu.cn.
² Department of Biology, East Carolina University, Greenville, NC 27858, USA. Electronic address: zhangb@ecu.edu.

PMID: 32828551
DOI: 10.1016/j.tig.2020.08.002

Abstract

CRISPR/Cas9 is a versatile tool for plant gene function studies and crop improvement. However, traditional CRISPR/Cas9-mediated genome editing requires plant tissue culture that is both time-consuming and genotype-dependent. Ma et al. recently reported a novel virus-based method for delivering CRISPR/Cas9 into plant cells, and this should further expand the application of CRISPR/Cas9-mediated genome editing.

Keywords: CRISPR/Cas9; crop improvement; gene function; genome editing; virus.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Comment

MeSH terms

CRISPR-Cas Systems*
Clustered Regularly Interspaced Short Palindromic Repeats
Gene Editing*
Genome, Plant
Plants / genetics