Monitoring antibody response following SARS-CoV-2 infection: diagnostic efficiency of 4 automated immunoassays

Diagn Microbiol Infect Dis. 2020 Nov;98(3):115140. doi: 10.1016/j.diagmicrobio.2020.115140. Epub 2020 Jul 12.

Abstract

Introduction: SARS-CoV-2 seroconversion is important for epidemiological studies as well as contact tracing.

Material and methods: The antibody response against SARS-CoV-2 was examined in 111 patients with a positive qRT-PCR. Seroconversion was assessed using the Elecsys from Roche, the Liaison S1/S2 IgG from Diasorin, the IgG and IgA from Euroimmun, as well as the VIDAS IgG and IgM. Specificity was estimated based on the measurement of SARS-CoV-2 antibodies in 96 residual samples collected during a non-pandemic period.

Results: The highest overall sensitivity for detecting seroconversion was obtained using the Elecsys (81.1%), the Euroimmun with a combined detection of IgG/IgA (86.5%), and the VIDAS with a simultaneous measurement of IgG/IgM (78.4%).The Elecsys and the VIDAS IgG/IgM demonstrated a specificity as well as a positive predictive value of 100%.

Conclusions: The Elecsys and the VIDAS methods with a combination of IgG/IgM measurement demonstrated a high sensitivity with no false positive results.

Keywords: SARS-CoV-2; immunoassays; seroconversion.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Antibodies, Viral / blood*
  • Antibodies, Viral / immunology
  • Antibody Formation / immunology
  • Betacoronavirus / immunology*
  • COVID-19
  • COVID-19 Testing
  • Clinical Laboratory Techniques*
  • Coronavirus Infections / diagnosis*
  • Female
  • Humans
  • Immunoassay / methods
  • Immunoglobulin A / blood
  • Immunoglobulin A / immunology
  • Immunoglobulin G / blood
  • Immunoglobulin G / immunology
  • Immunoglobulin M / blood
  • Immunoglobulin M / immunology
  • Male
  • Middle Aged
  • Pandemics
  • Pneumonia, Viral / diagnosis*
  • Polymerase Chain Reaction
  • SARS-CoV-2
  • Seroconversion
  • Serologic Tests / methods
  • Young Adult

Substances

  • Antibodies, Viral
  • Immunoglobulin A
  • Immunoglobulin G
  • Immunoglobulin M