Construction of the R17L mutant of MtC1LPMO for improved lignocellulosic biomass conversion by rational point mutation and investigation of the mechanism by molecular dynamics simulations

Xiao Guo; Yajing An; Chengcheng Chai; Jingcheng Sang; Luying Jiang; Fuping Lu; Yujie Dai; Fufeng Liu

doi:10.1016/j.biortech.2020.124024

Construction of the R17L mutant of MtC1LPMO for improved lignocellulosic biomass conversion by rational point mutation and investigation of the mechanism by molecular dynamics simulations

Bioresour Technol. 2020 Dec:317:124024. doi: 10.1016/j.biortech.2020.124024. Epub 2020 Aug 16.

Authors

Xiao Guo¹, Yajing An¹, Chengcheng Chai¹, Jingcheng Sang¹, Luying Jiang¹, Fuping Lu¹, Yujie Dai¹, Fufeng Liu²

Affiliations

¹ Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education, Tianjin 300457, PR China; Tianjin Key Laboratory of Industrial Microbiology, Tianjin 300457, PR China; College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, PR China.
² Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education, Tianjin 300457, PR China; Tianjin Key Laboratory of Industrial Microbiology, Tianjin 300457, PR China; College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, PR China. Electronic address: fufengliu@tust.edu.cn.

PMID: 32836036
DOI: 10.1016/j.biortech.2020.124024

Abstract

To enhance the biomass conversion efficiency, the R17L mutant of the lytic polysaccharide monooxygenase (LPMO) MtC1LPMO with improved catalytic efficiency was constructed via rational point mutation based on the HotSpot Wizard 3.0 and dezyme web servers. Compared with the wild-type (WT) MtC1LPMO, R17L exhibited a 1.8-fold increase of specific activity and 1.92-fold increase of catalytic efficiency (k_cat/K_m). The degree of increase of the reducing sugar yield from microcrystalline cellulose and three plant biomass materials during synergistic hydrolysis using cellulase in combination with R17L was about 2 times higher than with the WT. Molecular dynamics simulations revealed that the R17L mutation reduced the stability of the region R18-I36, which then weakened the direct interactions between region N24-V31 and the substrate cellohexaose. Consequently, the deflection time of the cellohexaose conformation in R17L was prolonged compared to the WT, which enhanced its catalytic efficiency.

Keywords: Catalytic activity; Lignocellulosic biomass; Lytic polysaccharide monooxygenase; Molecular dynamics simulations; Rational mutagenesis.

MeSH terms

Biomass
Lignin
Molecular Dynamics Simulation*
Point Mutation*

Substances

lignocellulose
Lignin