Chemical composition, antioxidant, antimicrobial and antiproliferative activities of the extracts isolated from the pomace of rowanberry (Sorbus aucuparia L.)

Ramunė Bobinaitė; Charlotte Grootaert; John Van Camp; Antanas Šarkinas; Mindaugas Liaudanskas; Vaidotas Žvikas; Pranas Viškelis; Petras Rimantas Venskutonis

doi:10.1016/j.foodres.2020.109310

Chemical composition, antioxidant, antimicrobial and antiproliferative activities of the extracts isolated from the pomace of rowanberry (Sorbus aucuparia L.)

Food Res Int. 2020 Oct:136:109310. doi: 10.1016/j.foodres.2020.109310. Epub 2020 May 17.

Authors

Ramunė Bobinaitė¹, Charlotte Grootaert², John Van Camp², Antanas Šarkinas³, Mindaugas Liaudanskas⁴, Vaidotas Žvikas⁵, Pranas Viškelis⁶, Petras Rimantas Venskutonis⁷

Affiliations

¹ Department of Food Science and Technology, Kaunas University of Technology, Radvilėnų, pl.19, LT-50254, Lithuania.
² Department of Food Technology, Safety and Health, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, Belgium.
³ Food Institute of Kaunas University of Technology, Radvilėnų pl. 19, LT-44239 Kaunas, Lithuania.
⁴ Institute of Pharmaceutical Technologies of the Faculty of Pharmacy of Lithuanian University of Health Sciences, Sukilėlių av. 13, LT-50162 Kaunas, Lithuania; Biochemistry and Technology Laboratory, Institute of Horticulture LAMMC, Kaunas st. 30, LT-54333 Babtai, Kaunas Distr., Lithuania.
⁵ Institute of Pharmaceutical Technologies of the Faculty of Pharmacy of Lithuanian University of Health Sciences, Sukilėlių av. 13, LT-50162 Kaunas, Lithuania.
⁶ Biochemistry and Technology Laboratory, Institute of Horticulture LAMMC, Kaunas st. 30, LT-54333 Babtai, Kaunas Distr., Lithuania.
⁷ Department of Food Science and Technology, Kaunas University of Technology, Radvilėnų, pl.19, LT-50254, Lithuania. Electronic address: rimas.venskutonis@ktu.lt.

PMID: 32846519
DOI: 10.1016/j.foodres.2020.109310

Abstract

In the present work, acetone, ethanol and water extracts of rowanberry (Sorbus aucuparia L.) pomace were evaluated for their antiproliferative, antimicrobial and antioxidative effects. Chemical composition of the extracts was determined by UPLC-ESI-MS/MS and spectrophotometric methods. Neochlorogenic and chlorogenic acids were the major phenolic compounds. The water extract contained the highest total proanthocyanidins content (301 ± 18.9 mg/g) and demonstrated the highest antioxidant activity in the all assays (DPPH, FRAP and ORAC). Extracts isolated from rowanberry pomace effectively inhibited the growth of undesirable microorganisms, especially Gram-positive bacteria. Acetone extract was the strongest antimicrobial agent followed by water and ethanol extracts. Acetone and water extracts demonstrated also higher cytotoxic potential in cell viability assays (SRB and MTT) using Caco-2 cells. In general, the results suggest that rowanberry pomace is a promising source of natural compounds with antioxidant and biological activities.

Keywords: Biological activity; Phenolic compounds; Rowanberry by-products; Solvent extraction.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Acetone
Anti-Infective Agents / pharmacology*
Antioxidants / chemistry
Antioxidants / pharmacology*
Bacteria / drug effects
Caco-2 Cells
Cell Proliferation / drug effects
Cell Survival / drug effects
Chlorogenic Acid / analysis
Ethanol
Fruit / chemistry
Humans
Plant Extracts / chemistry*
Plant Extracts / pharmacology*
Proanthocyanidins / analysis
Sorbus / chemistry*
Water

Substances

Anti-Infective Agents
Antioxidants
Plant Extracts
Proanthocyanidins
Water
Acetone
Chlorogenic Acid
Ethanol