Beneficial Effect of a Fermented Wheat Germ Extract in Intestinal Epithelial Cells in case of Lipopolysaccharide-Evoked Inflammation

Oxid Med Cell Longev. 2020 Aug 5:2020:1482482. doi: 10.1155/2020/1482482. eCollection 2020.

Abstract

In this study, the protective effect of a fermented wheat germ extract (FWGE) against LPS-induced inflammation and oxidative stress in IPEC-J2 porcine intestinal epithelial cells was studied. Enterocytes were treated with LPS derived from Salmonella enterica ser. Typhimurium and Escherichia coli O55:B5, O111:B4, and O127:B8 strains. Intracellular ROS level and extracellular H2O2 level were followed up by two fluorescent assays (DCFH-DA and Amplex Red). The effect of FWGE on the intestinal barrier integrity was determined by transepithelial electric resistance measurements and using a FD4 fluorescent tracer dye. IL-6 concentration of supernatants was also measured by the ELISA method. Our data revealed that FWGE had a significant lowering effect on the inflammatory response especially related to oxidative stress. Treatment with FWGE (1-2%) significantly decreased the level of intracellular ROS compared to LPS-treated cells. Furthermore, LPS-triggered partial disruption of epithelial integrity was reduced after FWGE application.

MeSH terms

  • Animals
  • Cell Line
  • Cell Survival / drug effects
  • Epithelial Cells / drug effects
  • Epithelial Cells / pathology*
  • Fluorescent Dyes / chemistry
  • Inflammation / pathology*
  • Interleukin-6 / metabolism
  • Intestines / pathology*
  • Intracellular Space / metabolism
  • Lipopolysaccharides
  • Permeability / drug effects
  • Plant Extracts / pharmacology*
  • Reactive Oxygen Species / metabolism
  • Swine

Substances

  • Avemar
  • Fluorescent Dyes
  • Interleukin-6
  • Lipopolysaccharides
  • Plant Extracts
  • Reactive Oxygen Species