Adult and endemic neurogenesis in the vestibular nuclei after unilateral vestibular neurectomy

Prog Neurobiol. 2021 Jan;196:101899. doi: 10.1016/j.pneurobio.2020.101899. Epub 2020 Aug 26.

Abstract

We previously revealed adult reactive neurogenesis in deafferented vestibular nuclei following unilateral vestibular neurectomy (UVN) in the feline model. We recently replicated the same surgery in a rodent model and aimed to elucidate the origin and fate of newly generated cells following UVN. We used specific markers of cell proliferation, glial reaction, and cell differentiation in the medial vestibular nucleus (MVN) of adult rats. UVN induced an intense cell proliferation and glial reaction with an increase of GFAP-Immunoreactive (Ir), IBA1-Ir and Olig2-Ir cells 3 days after the lesion in the deafferented MVN. Most of the newly generated cells survived after UVN and differentiated into oligodendrocytes, astrocytes, microglial cells and GABAergic neurons. Interestingly, UVN induced a significant increase in a population of cells colocalizing SOX2 and GFAP 3 days after lesion in the deafferented MVN indicating the probable presence of multipotent cells in the vestibular nuclei. The concomitant increase in BrdU- and SOX2-Ir cells with the presence of SOX2 and GFAP colocalization 3 days after UVN in the deafferented MVN may support local mitotic activity of endemic quiescent neural stem cells in the parenchyma of vestibular nuclei.

Keywords: Adult neurogenesis; Cell proliferation; Oligodendrogenesis; Stem cells; Vestibular compensation; Vestibular nuclei.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Behavior, Animal / physiology
  • Cell Proliferation / physiology*
  • Denervation
  • Male
  • Neural Stem Cells
  • Neurogenesis / physiology*
  • Oligodendroglia / physiology*
  • Rats
  • Rats, Long-Evans
  • Vestibular Diseases / physiopathology*
  • Vestibular Nuclei / physiology*
  • Vestibular Nuclei / surgery*