Miniaturized LC in Molecular Omics

Anal Chem. 2020 Sep 1;92(17):11485-11497. doi: 10.1021/acs.analchem.0c01436. Epub 2020 Aug 12.

Abstract

Miniaturized LC has evolved at an exponential rate over the last 50 years. In the past decade, it has received considerable attention in the field of bioanalytical separation science and technology due to the need to measure different classes of biomolecules present in a variety of matrixes on a global scale to gain a deeper understanding of complex biological processes. This field has become a dominant area underpinning the molecular omics research (e.g., proteomics, metabolomics, lipidomics, and foodomics), allowing key insights into the function and mechanism of small to very large biomolecules on a molecular level. This Feature highlights the recent advances in molecular omics focusing on miniaturized LC technology combined with mass spectrometry-based platforms, with a particular emphasis on the strategies adopted and applications using new and sensitive nanoscale analytical methodologies.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Amino Acids / analysis
  • Amino Acids / isolation & purification
  • Chromatography, Liquid / instrumentation*
  • Chromatography, Liquid / methods*
  • Humans
  • Lipids / analysis
  • Lipids / isolation & purification
  • Mass Spectrometry
  • Metabolomics
  • Nanostructures / chemistry*
  • Organic Chemicals / analysis
  • Organic Chemicals / isolation & purification
  • Proteomics
  • Surface Properties

Substances

  • Amino Acids
  • Lipids
  • Organic Chemicals