The use of a selective staphylococcal broth v direct plating for the recovery of Staphylococcus aureus

Infect Control Hosp Epidemiol. 1988 May;9(5):204-5. doi: 10.1086/645834.


Nine hundred seventy-two cultures taken from the external nares and the vaginal vestibules of 54 women for the isolation of Staphylococcus aureus were studied. The swabs were plated directly to a trypticase soy agar plate containing 5% sheep blood and were then placed into a selective staphylococcal broth. Both culture methods were compared for the ability to recover S aureus. Twenty percent (26/131) and 66% (38/58) of the S aureus-positive cultures taken from the nares and vagina respectively were cultured from the selective broth only. We believe that a selective staphylococcal broth should be used in addition to routine culture techniques to isolate S aureus from infection control surveillance cultures.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Bacteriological Techniques
  • Culture Media*
  • Female
  • Humans
  • Middle Aged
  • Nose / microbiology
  • Staphylococcus aureus / isolation & purification*
  • Vagina / microbiology


  • Culture Media