Expression of Ascorbate Peroxidase Derived from Cyanidioschyzon merolae in Mammalian Cells

In Vivo. 2020 Sep-Oct;34(5):2437-2441. doi: 10.21873/invivo.12058.


Background/aim: Ascorbate peroxidase (APX) derived from Cyanidioschyzon merolae, a primitive red alga living in high temperature and acidic environments, has greater anti-oxidative capacity than similar peroxidases occurring in other plants. In the present study, we examined whether expression of Cyanidioschyzon merolae-derived APX (cAPX) in mammalian cells increases cellular anti-oxidative capacity.

Materials and methods: The cAPX gene was introduced into the mouse fibroblast-like cell line C3H10T1/2. Production of reactive oxygen species (ROS) and/or cell viability was assessed after heat, H2O2 and acid stimulation.

Results: Heat and H2O2 stimulation resulted in ROS production. cAPX-expressing cells were more tolerant to oxidative stress induced by heat, H2O2 and acid stimulations than control cells lacking cAPX.

Conclusion: Introduction of cAPX increases the anti-oxidative capacity in mammalian cells.

Keywords: Ascorbate peroxidase; Cyanidioschyzon merolae; anti-oxidative stress; fibroblast; reactive oxygen species.

MeSH terms

  • Animals
  • Ascorbate Peroxidases / genetics
  • Ascorbate Peroxidases / metabolism
  • Cytosol / metabolism
  • Gene Expression Regulation, Plant
  • Hydrogen Peroxide* / pharmacology
  • Mice
  • Rhodophyta* / genetics
  • Rhodophyta* / metabolism


  • Hydrogen Peroxide
  • Ascorbate Peroxidases