Droplet-Microarray: Miniaturized Platform for High-Throughput Screening of Antimicrobial Compounds

Adv Biosyst. 2020 Oct;4(10):e2000073. doi: 10.1002/adbi.202000073. Epub 2020 Sep 2.


Currently, there are no time-saving and cost-effective high-throughput screening methods for the evaluation of bacterial drug-resistance. In this study, a droplet microarray (DMA) system is established as a miniaturized platform for high-throughput screening of antibacterial compounds using the emerging, opportunistic human pathogen Pseudomonas aeruginosa (P. aeruginosa) as a target. Based on the differences in wettability of DMA slides, a rapid method for generating microarrays of nanoliter-sized droplets containing bacteria is developed. The bacterial growth in droplets is evaluated using fluorescence. The new method enables immediate screening with libraries of antibiotics. A novel simple colorimetric readout method compatible with the nanoliter size of the droplets is established. Furthermore, the drug-resistance of P. aeruginosa 49, a multi-resistant strain from an environmental isolate, is investigated. This study demonstrates the potential of the DMA platform for the rapid formation of microarrays of bacteria for high-throughput drug screening.

Keywords: antibiotic resistance screening; droplet-microarray; facultative pathogenic Pseudomonas aeruginosa.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Colony Count, Microbial / methods*
  • Equipment Design
  • High-Throughput Screening Assays / methods*
  • Humans
  • Microbial Sensitivity Tests / methods*
  • Pseudomonas Infections / microbiology
  • Pseudomonas aeruginosa / drug effects


  • Anti-Bacterial Agents