Effects of cadmium on two wheat cultivars depending on different nitrogen supply

Abstract

Heavy metal pollution as well as improper fertilization management represent serious threats to a clean environment and healthy food. This study was conducted to investigate how nitrogen supply influences a plant's ability to cope with cadmium stress in the two wheat cultivars - the modern cv. Katya (carrier of the semi-dwarfing gene Rht8) and the old cv. Slomer. Here we examined the effects of 100 μM CdCl₂ on both wheat genotypes grown hydroponically under three different nutrition regimes of 5.5, 10 and 20 mM NO₃^- by investigating plant growth, pigment content and the functional activity of the photosynthetic apparatus through a combination of PAM chlorophyll fluorescence, P700 photooxidation, oxygen evolution and oxidative stress markers. Data showed that the different genetic background affects the different strategies for metal uptake and allocation, as well as abilities to deal with oxidative stress. The modern cv. Katya restricts the entry of the metal to the roots, but allows its translocation to the shoots. Nevertheless, the photosynthetic performance indicated better protection, possibly mediated by the Rht8 allele. In contrast, the old cv. Slomer tolerates higher cadmium levels in roots and possesses efficient barriers against its transfer to the shoots, but still showed more impaired photosynthetic activity. In general, the impact of cadmium on the photosynthetic apparatus was most deleterious under the lowest nitrogen concentration which was applied, while the highest nitrogen supply alleviated the negative effects of cadmium. The data suggest that the modern breeding allele (Rht8), as well as a better nutrition might contribute to the tolerance to heavy metal stress in the wheat.

Keywords: Cadmium; Nitrogen; Oxidative stress; Oxygen evolution; Photosynthetic apparatus; Pigments; Wheat cultivars.