Luteolin Induces Cytotoxicity in Mix Cellularity Classical Hodgkin's Lymphoma via Caspase Activated-cell Death

Anticancer Res. 2020 Sep;40(9):4907-4912. doi: 10.21873/anticanres.14493.

Abstract

Background/aim: We investigated the effects of luteolin (LUT) on classical Hodgkin's lymphoma (cHL), since such studies in malignant lymphomas are lacking.

Materials and methods: Effect of LUT on cell growth was assessed with water-soluble tetrazolium 1 (WST-1) cell proliferation assay and automated hemocytometry on trypan blue-exclusion assay. Cell death was investigated with acridine orange/ethidium bromide live-dead assay, propidium iodide (PI) flow cytometry, and Annexin-V-PI microscopy. Caspase activation was studied using CellEvent Caspase-3/7 Green detection reagent. High resolution immunofluorescence microscopy was used to detect cleaved-PARP-1.

Results: LUT induced a dose-dependent decrease in the growth of KMH2 and L428 cells, cellular models of mix-cellularity (MC) and nodular sclerosis (NS) cHL, respectively. However, LUT induced cell death only in KMH2, at a higher concentration, and this was associated with caspase activation and cleaved PARP-1.

Conclusion: LUT induces cytotoxicity in the MC-cHL cellular model KMH2 via caspase activation.

Keywords: Hodgkin's lymphoma; Luteolin; cancer; mix cellularity classical Hodgkin's lymphoma.

MeSH terms

  • Antineoplastic Agents / pharmacology*
  • Apoptosis / drug effects
  • Caspases / metabolism*
  • Cell Death / drug effects*
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Hodgkin Disease / drug therapy
  • Hodgkin Disease / pathology
  • Humans
  • Luteolin / pharmacology*
  • Poly (ADP-Ribose) Polymerase-1 / metabolism

Substances

  • Antineoplastic Agents
  • PARP1 protein, human
  • Poly (ADP-Ribose) Polymerase-1
  • Caspases
  • Luteolin